Jun 01, 2018 · The key difference between Pour plate and Spread plate is that a known volume of the sample is spread on the surface of the agar medium in spread plate, while, in pour plate, a known volume of the sample is mixed with agar and then poured into a plate. When comparing the accuracy of these two techniques, pour plate has a higher accuracy than the spread plate.
Direct and Viable Microbial Count Methods Basic Jan 03, 2020 · For accurate counts, the optimum count should be within the range of 30- 300 colonies/plate. To ensure a countable plate a series of dilutions should be plated. Disadvantages of Pour plate method. Preparation for the pour plate method is time consuming compared with streak plate/and or spread plate technique.
May 19, 2010 · On a streaked plate there may be many different species. The single colonies of interest are then sub-cultured in order to obtain pure, confluent growth to show that the follow-up biochemistry was performed on a pure culture otherwise the biochemistry results cannot be trusted and the procedure would need to be repeated.
Microbiology Lab :MOLB 2210Know the difference between a Gram-positive cell wall and a Gram-negative cell wall. Dont forget to note the presence of LPS in Gram-negative cells and teichoic acid in Gram-positive cells. A pour plate, a spread plate and a streak plate are all methods used to derive pure cultures. A pure culture is a culture that is derived from 1
Pour plate Method:Principle, Procedure, Uses, and (Dis The pour plate method of counting bacteria is more precise than the streak plate method, but, on the average, it will give a lower count as heat sensitive microorganisms may die when they come contact with hot, molten agar medium. Uses:The pour plate technique can be used to determine the number of microbes/mL in a specimen.
The streak plate method is a rapid qualitative isolation method. The techniques commonly used for isolation of discrete colonies initially require that the number of organisms in the inoculums be reduced. It is essentially a dilution technique that involves spreading a loopful of culture over the surface of an agar plate.
Streak Plate Method- Principle, Methods, Significance Feb 19, 2020 · Streak Plate Method- Principle, Methods, Significance, Limitations. In microbiology, streaking is a technique used to isolate a pure strain from a single species of microorganism, often bacteria. The dilution or isolation by streaking method was first developed by Loeffler and Gaffky in Kochs laboratory, which involves the dilution of bacteria by systematically streaking them over the
Streak plate method:Principle, Purpose, Procedure, and Jul 16, 2016 · The sample/inoculum is diluted by streaking it across the surface of the agar plate. While streaking in successive areas of the plate, the inoculum is diluted to the point where there is only one bacterial cell deposited every few millimeters on the surface of the agar plate.
Feb 13, 2010 · For a streak plate you streak the sample across one edge of the plate using the swab if that is how the sample came, or a sterile loop if the sample is a liquid or spreadable slurry or paste. Then take one streak from that, another from that and another from that, sterilising the loop in a flame betwen each streak, giving four different